EZH2 (A677T)/EED/SUZ12/RbAp48/AEBP2, His-tag, FLAG-tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: 2-end (all components)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.

Description: Mutant version of BPS regular EZH2 5-member complex (Cat. # 51004), but with a Ala-to-Thr mutation on a.a. 682 of the EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), (a.a. 2-end; A682T) with N-terminal His tag, MW= 86 kDa, human EED (NM_003797) (a.a. 2-end) with N-terminal Flag tag, MW= 51 kDa, human SUZ12 (NM_015355) (a.a. 2-end) with N-terminal His tag, MW = 87 kDa, Human AEBP2 (NM_153207) (a.a. 2-end) with N-terminal His tag, MW= 53 kDa, and human RbAp48 (NM_005610) (a.a. 2-end) with N-terminal His tag, MW = 48 kDa, co-expressed in baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.

Genbank: NM_004456 (EZH2), NM_003797(EED), NM_015355 (SUZ12), NM_153207(AEBP2), NM_005610(RbAP48)

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag (EZH2, SUZ12, RbAp48,AEBP2), N-terminal FLAG-tag (EED)

Uniprot: Q15910

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Varambally, S. et al. Science. 2008 322(5908): 1695-1699.
2. Rakotobe, D. et al., Virol. J. 2010 5, 32.Application Reference: Morin, R.D., et al. Nat Genet. 2010 February; 42(2): 181-185.

• Artikelnummer: BPS51039
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 51039
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Wirt: Insect