EZH2 (A738T) Chemiluminescent Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and HTS applications.

Description: The EZH2 (A738T) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (A738T)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The EZH2 (A738T) Chemiluminescent Assay Kit comes in a convenient format, with wells precoated with histone H3 peptide substrate, an antibody against methylated K27 residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified EZH2 (A738T) complex for 100 enzyme reactions. The key to the EZH2 (A738T) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Storage Stability: One year from date of receipt when stored as directed.

Supplied As: Kit comes in a convenient format, with wells precoated with histone H3 peptide substrate

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Dillon, S.C., et al. Genome Biology 2005; 6:227. 2. Morin, R.D., et al. Nat Genet. 2010, 42(2):181.

• Artikelnummer: BPS52067
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 52067
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK