FAP, His-Tag Recombinant

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Encompassing Amino Acids: 29-end

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 50 mM Tris-HCl (pH 7.5),10 mM MgCl₂, 1 mM MnCl₂, 5 µMsubstrate (A-P-AMC), incubation 10 min atroom temperature.

Background: Fibroblast Activation Protein, or FAP, is a 170 kDa dimer withdipeptidase activity. FAP expression is seen on activated stromal fibroblasts in >90% ofall human carcinomas, suggesting a role in the development, growth and metastasis ofcarcinomas. It is also involved in the control of wound healing and tissue repair.

Description: Human FAP (GenBank Accession No. NM_004460), (a.a. 29-end) with C-terminal His-tag, MW=85.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.

Genbank: NM_004460

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal His-tag

Uniprot: Q12884

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Edosada,C.Y. et al., J. Biol. Chem. 281 (11), 7437-7444 (2006).
2. Edosada,C.Y. et al., FEBS Lett. 580 (6), 1581-1586 (2006).

• Artikelnummer: BPS80100
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80100
• Green Labware: Nein
• Verpackungseinheit: 25 µg
• Mengeneinheit: STK