FBXL11 (KDM2A, JHDM1A), FLAG-Avi-tags Recombinant

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Encompassing Amino Acids: 2-700

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: The assay wasperformed using BPS BioscienceFBXL11 Homogeneous Assay Kit (Cat#50611) and following themanufacturer's recommended protocol.

Description: Human F-Box Protein 11 (FBXL11), alsoknown as KDM2A, Ndy2, and JHDM1AGenBank Accession # NM_012308, a.a. 2-700 with N-terminal FLAGAvi-tag, MW = 82 kDa, expressed in Sf9cells via a baculovirus expressionsystem.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween-20,20% glycerol, 80 µg/ml FLAG peptide

Genbank: NM_012308

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal FLAG-Avi-tags

Uniprot: Q9Y2K7

Warnings: Avoidfreeze/thaw cycles. Storing diluted enzyme isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).

Biosafety Level: Not applicable (BSL-1)

References: 1. Tanaka, Y., et al. (2010). EMBO J. 29(9):1510-22.
2. Blackledge, N.P., et al. (2010). Mol. Cell. 38(2):179-90.
3. Frescas, D., et al. (2008). Cell Cycle 7(22):3539-47.

• Artikelnummer: BPS50156
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50156
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Wirt: Insect