Fc (IgG1): FcRn Inhibitor Screening TR-FRET Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Application: Screen or titrate inhibitors of FcRn binding to Fc (IgG1).

Background: Neonatal Fc receptor for IgG (FcRn) is a heterodimeric protein. FcRn consists of the Fc Gamma Receptor and Transporter encoded by the FCGRT gene, associated with beta-2-Microglobulin (B2M). FcRn binds to the Fc region of monomeric immunoglobulin G (IgG). It is expressed in over 25 tissue types, with high expression levels observed in the spleen and intestine. In the placenta, it transports IgGs from mother to fetus. FcRn contributes to an effective humoral immunity by protecting IgGs from degradation, recycling them and extending their half-life in circulation. In addition to IgGs, it regulates the homeostasis of serum albumin. FcRn is a potential therapeutic target for autoimmune diseases. Disrupting the FcRn/IgG interaction is expected to increase the overall clearance of IgGs, including disease-causing autoantibodies. Engineered Fc fragments or neutralizing IgGs that bind to FcRn with high affinity through their Fc region are currently undergoing clinical trial. The first FDA-approved drug targeting FcRn (efgartigimod) is now used to treat myasthenia gravis, an autoimmune neuromuscular disease caused by the presence of autoantibodies against acetylcholine receptor, providing proof-of-concept in favor of this strategy.

Contraindications: This kit is compatible with up to 1% final DMSO concentration. Compounds that are fluorescent may interfere with the results, depending on their spectral excitation and emission properties.It is recommended that the compound alone is tested to determine any potential interference of the compound on the assay results.

Description: The Fc (IgG1): FcRn Inhibitor Screening TR-FRET Assay Kit is designed for screening and profiling of neutralizing antibodies or inhibitors of the interaction between Fc (IgG1) and human FcRn (Neonatal Fc receptor for IgG) using TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer). This kit requires no time-consuming washing steps and comes in a convenient 384-well format, with purified Biotinylated-FcRn complex (Fc receptor amino acids 24-297 and B2M amino acids 21-119) and europium-labeled (Eu) Fc (IgG1) protein (amino acids 100-330), Dye-labeled Acceptor, and assay buffer for 400 reactions.The assay procedure is straightforward and simple; a sample containing europium-labeled (Eu) Fc (IgG1), dye-labeled acceptor, biotin-labeled FcRn, and an inhibitor is incubated for one hour. Then, the fluorescence signal is measured at 620 nm and 665 nm using the TR-FRET module of a fluorescent microplate reader. When FcRn binds to Fc (IgG1), energy transfer occurs between the europium donor and the dye-labeled acceptor, producing a fluorescent signal. Inhibitors that block this interaction prevent energy transfer, reducing the fluorescent signal.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Uniprot: P55899

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

References: Dall'Acqua W.F., et al. 2002 J Immunol. 169(9): 5171-80.

• Artikelnummer: BPS82653
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82653
• Verpackungseinheit: 384 reactions
• Mengeneinheit: PAK