FcRn (FCGRT/B2M) Blocker

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Applications: This product is for research use only. It is not suitable for human diagnostic or therapeutic use.

Assay Conditions: Assay 1. A 96-well plate was coated overnight at 4°C with 50 µl/well of the FcRn blocker at a concentration of 2 µg/ml, diluted in PBS. The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked with 100 µl of Blocking Buffer 6 for an hour at room temperature. A serial dilution of biotinylated FcRn protein (BPS Bioscience #71283) prepared in Blocking Buffer 6 was added for 30 minutes. After 3 washes, the plate was incubated with Streptavidin-HRP followed by more washes and addition of a colorimetric HRP substrate. After quenching, color intensity was measured at 450 nm.Assay 2. The effect of FcRn (FCGRT/B2M) Blocker was assessed using the Fc(IgG1):FcRn Inhibitor Screening colorimetric kit (BPS Bioscience #78501) using the following conditions: Fc(IgG1) protein at 2 µg/ml, diluted in PBS, serial dilution of FcRn Blocker prepared in blocking buffer, and 1.5 µg/ml of FcRn-Biotin.

Description: The FcRn (FCGRT/B2M) blocker (Fc-Tag) is a purified human protein corresponding to the Fc region of an antibody which has been engineered to bind to human neonatal Fc Receptor for IgG (FcRn; FCGRT/B2M) with high affinity. This antibody has been tested for specific binding to human FcRn (BPS Bioscience #71283) and it blocks the binding of FcRn with immunoglobulins G.

Format: Aqueous buffer solution 

Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Host Cell Line: HEK293

Purification: Protein A affinity

Purity: ≥90%

Storage Stability: At least 12 months at -80°C. 

Uniprot: P55899

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101468-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101468-2
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Wirt: Human