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Encompassing Amino Acids: 447-761
Assay Conditions: Assay was done in Kinase buffer containing 2 mM DTT using 0.2 mg/mL Poly-(Glu:Tyr) substrate and 10 µM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Description: Dephosphorylated recombinant FGFR3 (Fibroblast Growth Factor Receptor 3) encompassing amino acids 397-806. This protein contains an N-terminal His-Tag and has been biotinylated using an N-terminal Avi-Tag™. The recombinant protein is purified to ≥90%
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT.
Genbank: NM_000142
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal His-Avi-tag
Uniprot: P22607
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Cappellen, D. et al., Nature Genetics. (1999); 23:18-20.
2. Bellus, G.A. et al., AM. J. Hum. Genet. (1995); 56(2):368-373.