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Encompassing Amino Acids: 397-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate FGFR3 with 0.25 mg/ml Poly (Glu:Tyr, 4:1) peptide substrate and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with ascintillation counter.
Background: Fibroblast growth factor receptor 3 (FGFR3) is part of a family of fibroblast growth factor receptors that share similar structures and functions. FGFR3 plays a role in several important cellular processes, including regulation of cell growth and division, determination of cell fate, formation of blood vessels, wound healing and embryo development (1). FGFR3 is involved in the development and maintenance of bone and brain tissue. Mutations in FGFR3 have been implicated in causing bladder cancer, cancer of white blood cells (multiple myeloma) and cervical cancer (2).
Description: Human FGFR3, (GenBank Accession #NM_000142) with N-terminal GST-tag, a.a 397-end, MW= 73 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_000142
Host Cell Line: Sf9 cells
Purity: ≥95%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P22607
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Chen, L. and Deng, C.X. Roles of FGF signaling in skeletal development and human genetic diseases. Front Biosci. 2005; 1(10):1961-1976.
2. Mhawech-Fauceglia, P. et al. 2006. FGFR3 and p53 protein expressions in patients with pTa and pT1 urothelial bladder cancer. Eur. J. Surg. Oncol. 2006; 32(2):231-237