FGL1:LAG3 Inhibitor Screening Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Screen for inhibitors of LAG3 binding to FGL1.

Background: Lymphocyte-activation gene 3 (LAG3, also known as CD223) is a cell surface receptor that negatively regulates the activation and proliferation of T cells. Fibrinogen-like protein 1 (FGL1), a liver-secreted protein present in the plasma, is a functional LAG3 ligand. Blockade of the FGL1-LAG3 interaction has been proposed as a therapeutic strategy to promoting antitumor responses in cancer patients.

Contraindications: Green and blue dyes, such as Trypan Blue, absorb light in the AlphaScreen™ signal emission range (520-620 nm). Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen™ assays.

Description: The FGL1:LAG3 Inhibitor Screening Assay is an AlphaLISA®-based assay designed to measure the inhibition of LAG3 binding to FGL1 in a homogeneous 384-reaction format. The assay is straightforward: first, FGL1 and LAG3 are incubated with or without a compound of interest. Then, acceptor and donor beads are added to the reaction, followed by reading the Alpha-counts.Illustration of the assay principle.
FGL1 contains a His tag recognized by the Nickel chelate acceptor bead, whereas LAG3 is biotinylated, allowing its binding to the streptavidin-coated acceptor bead. Interaction between FGL1 and its receptor LAG3 brings the donor and acceptor beads in proximity. A singlet oxygen generated upon excitation of the donor bead leads to the excitation of the acceptor bead, which emits light. Light emission in the assay is proportional to the level of interaction. AlphaLISA™ immunoassays are a no-wash alternative to ELISA. These assays are robust, highly amenable to high throughput applications, and ideal for a minimal hands-on approach.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Supplied As: The FGL1:LAG3 Inhibitor Screening Assay Kit contains sufficient amounts of purified recombinant FGL1 and LAG3 proteins, and assay buffer for 400 reactions.

Uniprot: Q08830

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS78824
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78824
• Green Labware: Nein
• Verpackungseinheit: 384 rxns.
• Mengeneinheit: PAK