Description: This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Collagen I (COL1A1) (NM_000088) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF, and 1mM Na3VO4).
Presentation: Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Protein Tag: Myc-DDK (Flag)
Protein Type: Over-Expression Lysate
Reconstitution Instructions: Reconstitute with SDS Sample Buffer
Purification: Unpurified
Predicted Molecular Weight: 139.01 kDa
Synonyms: COL1A1, Alpha-1 type I collagen, Collagen alpha 1 chain type I, Collagen alpha-1(I) chain, Collagen, type I, alpha 1, Collagen I, Pro-alpha-1 collagen type 1, OI4