Presentation: SDS sample buffer containing 50 mM DDT
Description: Heat inactivated Skeletal Muscle Lysate sourced from Human Skeletal Muscle Tissue product is a tissue lysate which was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.
Concentration: 2 mg/ml
Recommended Storage: Short term: Store at 2-8°C. Long term: Aliquot and freeze at -70°C for up to 6 months. Avoid freeze-thaw cycles.
Protein Type: Cell/Tissue Lysate