BOB.1 (B-Cell Marker)(BOB1/2421), 0.2mg/mL

Description: BOB-1 is a transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2, boosting the promoter activity of those complexes. It is essential for the response of B-cells to antigens, required for the formation of germinal centers, and regilates IL-6 expression. BOB-1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB-1. Conversely, only some cases of classical Hodgkin lymphoma show BOB-1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB-1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Conjugate: Purified, with BSA

Concentration: 0.2 mg/mL

Storage buffer: PBS, 0.05% BSA, 0.05% azide

Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Clone: BOB1/2421

Entrez Gene ID: 5450

Immunogen: Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)

Verified AB Applications: Flow (intracellular) (verified)/IHC (FFPE) (verified)/WB (verified)

Z-Antibody Applications: Flow, intracellular (verified)/IHC, FFPE (verified)/WB (verified)

• Artikelnummer: BTMBNUB2421-100
• Hersteller: Biotium
• Hersteller Artikelnummer: BNUB2421-100
• Verpackungseinheit: 100 uL
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Monoclonal
• Methode: Western Blotting, Flow Cytometry, Immunohistochemistry
• Isotyp: IgG2b kappa
• Wirt: Mouse