Specificity: Recognizes endogenous levels of Collagen 10 alpha 1 protein.
Antibody Modification: Unconjugated
Presentation: 0.42% Potassium Phosphate, pH 7.3, 0.87% NaCl, 0.01% Sodium Azide, 30% Glycerol
Immunogen: Recombinant full length protein of human Collagen 10 alpha 1
Gene: COL10A1 / Collagen X
Description: Collagen X antibody LS-C667746 is an unconjugated rabbit polyclonal antibody to Collagen X (COL10A1) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Cited in 1 publication.
Usage: Western blot analysis of Collagen 10 alpha 1 expression in MCF7 (A) whole cell lysates. Immunohistochemical analysis of Collagen 10 alpha 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Synonyms: COL10A1, Collagen alpha-1(X) chain, Collagen, type X, alpha 1
Recommended Storage: Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.