MHC II (HLA-DRA)(IPO-10), 1mg/mL

Description: MAb IPO-10 defines the antigen that appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50% of cells). The MAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This MAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70% of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkin's disease cases a significant percentage of affected lymph node cells were detected. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Conjugate: Purified, BSA-free

Concentration: 1 mg/mL

Storage buffer: PBS, no BSA, no azide

Product Origin: Animal - Mus musculus (mouse)

Clone: IPO-10

Entrez Gene ID: 3122

Immunogen: Spleen cells of a patient with hairy cell leukemia (Daudi cells)

• Artikelnummer: BTMBNUM1090-50
• Hersteller: Biotium
• Hersteller Artikelnummer: BNUM1090-50
• Green Labware: Nein
• Verpackungseinheit: 50 uL
• Mengeneinheit: STK
• Reaktivität: Human, Monkey (Primate)
• Klonalität: Monoclonal
• Isotyp: IgG3 kappa
• Wirt: Mouse