Cdk2 Isoform 1 (human, recombinant)

Formulation: Lyophilized from sterile 50 mM Tris, pH 8.0, with 100 mM sodium chloride and 10% glycerol

Purity: ≥85% estimated by SDS-PAGE, ≥95% determined by SEC-HPLC

Formula Weight: 0

Shelf life (days): 365

Notes: Cyclin-dependent kinase 2 (Cdk2) is a serine/threonine kinase and member of the CDK family of kinases involved in cell cycle regulation.{15254,51716} It is composed of an N-terminal domain, an activation segment, also known as the T loop, and a catalytic C-terminal domain.{51716} Alternative splicing of CDK2 mRNA produces a full-length isoform or a shorter isoform that lacks exon 5, which encodes for part of the Cdk2 activation segment and contains residues that participate in interactions with cyclins.{51717} It is ubiquitously expressed and localizes to the cytoplasm or nucleus depending on the cell cycle stage.{51718,51719} Cdk2 associates with cyclin E to mediate the G1/S transition and with cyclin A during S phase progression.{15254,51716} Knockout of Cdk2 induces gametogenesis defects in mice but does not affect mitotic cell division.{51715} Protein levels of Cdk2 are increased in patients with oral squamous cell carcinoma, and overexpression of Cdk2/cyclin A or Cdk2/cyclin E is associated with poor prognosis in patients with oral cancer.{51714} Cayman’s Cdk2 Isoform 1 (human, recombinant) protein consists of 308 amino acids and has a calculated molecular weight of 35 kDa.

• Artikelnummer: CAY40793-500
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 40793-500
• Verpackungseinheit: 500 µg
• Mengeneinheit: STK
• Wirt: Cell Line