Akt1 (human, recombinant)

Formulation: 50 mM HEPES, pH 8.0, with 150 mM sodium chloride, 1 mM DTT, and 10% glycerol

Purity: ≥95% estimated by SDS-PAGE

Shelf life (days): 365

Notes: Akt1, also known as protein kinase Bα (PKBα), is a serine/threonine kinase belonging to the AGC kinase family and one of three Akt isoforms in mammals.{57326,59549} Akt kinases function downstream of activated tyrosine kinases and PI3K to regulate a variety of cellular processes, including cell size, growth, proliferation, and survival, as well as genome stability, glucose metabolism, and neovascularization.{59549} Akt1, like Akt2 and Akt3, is composed of an N-terminal pleckstrin homology (PH) domain, which binds to phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and phosphatidylinositol-(3,4)-diphosphate (PIP2), a kinase domain, and a C-terminal regulatory hydrophobic motif. It is ubiquitously expressed and is the primary isoform in endothelial cells.{15560} Akt1 is activated via recruitment to the plasma membrane, which is mediated by the interaction of the Akt1 PH domain with PI3K-generated PIP3 and PIP2, and subsequent phosphorylation at threonine 308 and serine 473.{59549} Increased Akt1 kinase activity has been found in tumor tissue isolated from patients with prostate, breast, and ovarian cancers.{59550} Cayman’s Akt1 (human, recombinant) protein was phosphorylated by recombinant 3-phosphoinositide-dependent kinase 1 (PDK1) and can be used for enzyme activity assays.

• Artikelnummer: CAY10011209
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 10011209-25
• Green Labware: Nein
• Verpackungseinheit: 25 µg
• Mengeneinheit: STK