cGAS (human, recombinant)

Formulation: 50 mM HEPES, pH 8.0, with 300 mM sodium chloride, and 10% glycerol

Purity: ≥90% estimated by SDS-PAGE

Shelf life (days): 365

Notes: Cyclic GMP-AMP (cGAMP) synthase (cGAS) is a nucleotidyltransferase located in the cytosol that acts as a cytosolic DNA sensor to detect foreign DNA from microbial pathogens as part of the innate immune response.{22400,22401} Upon binding to cytosolic DNA, cGAS produces the cyclic dinucleotide second messenger cGAMP, which activates stimulator of interferon genes (STING), leading to activation of the type I interferon (IFN) pathway.{22400,22401,39161} In vitro, fibroblasts, macrophages, and dendritic cells isolated from cGAS knockout (cGAS-/-) mice do not produce type I IFNs following DNA transfection or DNA virus infection.{39163} Similarly, cells containing a frame-shift mutation in the cGAS locus fail to mount an immune response to HIV and other retroviruses.{39164} In vivo, cGAS-/- mice infected with herpes simplex virus 1 (HSV-1) have lower levels of IFN-α and IFN-β, shorter survival times, and higher post-mortem levels of HSV-1 in the brain.{39163}

• Artikelnummer: CAY22810
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 22810-100
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Methode: Immunofluorescence, Immunoprecipitation, Western Blotting, ELISA, Flow Cytometry, Electron microscopy (EM), Electrophoresis, Mass Spectrometry, Cell-based Assay, Fluorescence, High-Throughput Screening