COX-1 (human, recombinant; mammalian expressed)

Formulation: 80 mM Tris, pH 8.0, with 0.3 mM diethyldithiocarbamic acid (DDC), 0.01% Tween 20, and 10% glycerol

Purity: ≥80% estimated by SDS-PAGE

Shelf life (days): 365

Notes: Cyclooxygenase 1 (COX-1) is a bifunctional enzyme that exhibits both COX and peroxidase activities.{500,501} It is composed of an N-terminal signal peptide, an EGF-like domain, a membrane binding domain, a catalytic domain, and a C-terminal tail.{4376} COX-1 is constitutively expressed in the gastrointestinal tract, kidney, spleen, liver, and lung and localizes to the endoplasmic reticulum.{1299,1394} The COX component converts arachidonic acid (Item Nos. 90010 / 90010.1 / 10006607) to a hydroperoxyl endoperoxide prostaglandin G2 (PGG2; Item No. 17010) and the peroxidase component reduces the endoperoxide to the corresponding alcohol PGH2 (Item No. 17020), the precursor of PGs, thromboxanes, and prostacyclins.{500,501} COX-1 is the target of many non-steroidal anti-inflammatory drugs (NSAIDs) and is responsible for the undesirable gastrointestinal and renal side effects, such as ulcer formation and reductions in the glomerular filtration rate, respectively.{5282,4411} Cayman’s COX-1 (human, recombinant; mammalian expressed) protein can be used for enzyme activity assays.

• Artikelnummer: CAY26306
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 26306-5
• Green Labware: Nein
• Verpackungseinheit: 5 Kunits
• Mengeneinheit: STK