Fatty Acid Desaturase 2 (human recombinant)

Formulation: 50 mM HEPES, pH 8.0, with 150 mM sodium chloride and 10% glycerol

Purity: ≥95% estimated by SDS-PAGE

Shelf life (days): 365

Notes: Fatty acid desaturase 2 (FADS2), also known as Δ6-desaturase, is a 444-amino acid transmembrane protein encoded by FADS2.{49056} This product is the N-terminal cytoplasmic domain of FADS2, corresponding to residues 2-131 of the full-length sequence. It catalyzes the rate-limiting step in the conversion of the ω-6 and ω-3 precursors linoleic acid (Item Nos. 90150 / 21909) and α-linolenic acid (Item No. 90210) to their respective long-chain polyunsaturated fatty acids (LC-PUFAs), inserting a cis double bond at position 6 of the fatty acid chain to convert linoleic acid to γ-linolenic acid (Item No. 90220) and α-linolenic acid to stearidonic acid (Item No. 90320), respectively. Following secondary desaturation by FADS1 (Item No. 25827) and chain elongation, FADS2 catalyzes desaturation of 24-carbon LC-PUFA intermediates. FADS2 also catalyzes the desaturation of palmitic acid (Item No. 10006627) to sapienic acid in human sebaceous glands and in various cancer cell lines that express FADS2.{45162,43766} Hepatic FADS2 protein levels and mRNA expression are increased in mice with high-fat diet-induced obesity and non-alcoholic steatohepatitis (NASH).{43213} FADS2 knockout (fads2-/-) mice are infertile, resistant to obesity, and have altered subcellular membrane diacylglycerol composition which leads to dysregulated post-translational processing of SREBP1c, a key transcription factor in the regulation of lipid metabolism.{45163}

• Artikelnummer: CAY25828
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 25828-100
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Methode: Immunoprecipitation, Western Blotting