IKKβ (human, recombinant)

Formulation: Active recombinant human N-terminal GST-tagged IKKβ expressed in insect cells

Purity: ≥80% estimated by SDS-PAGE

Shelf life (days): 180

Notes: IĸB kinase β (IKKβ) is a catalytic subunit of the IĸB kinase (IKK) complex, a key mediator of NF-κB activation.{57847} It is composed of an N-terminal kinase domain, which contains an activation loop with two serine residues, Ser 177 and Ser 181, that are subject to phosphorylation, a ubiquitin-like domain, a scaffold dimerization domain, and a C-terminal domain that binds the regulatory subunit IKKγ, also known as NF-κB essential modifier (NEMO). IKKβ is ubiquitously expressed and localizes in the cytoplasm to the IKK complex, which mediates NF-κB activation in response to numerous stimuli, including inflammatory cytokines and B- and T cell receptor engagement, as well as LPS and growth factors. Upon cellular stimulation, the TAK1-TAB complex phosphorylates and activates IKKβ, which induces phosphorylation and degradation of the NF-κB inhibitory protein IĸB, permitting NF-κB nuclear translocation and the transcription of numerous genes involved in inflammation, immunity, and cell survival and proliferation.{57848,57847} IKKβ has roles in several pathological conditions, including cancer and inflammatory diseases, such as rheumatoid arthritis.{57847} IKBKB mutations have been found in patients with severe combined immunodeficiency (SCID).{57849} Cayman’s IKKβ (human, recombinant) protein can be used for enzyme activity assays.

• Artikelnummer: CAY33739
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 33739-10
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK