JAK2 JH1 and JH2 Domains (human, recombinant; aa 532-1,132)

Formulation: 45 mM Tris-HCl, pH 8.0, with 300 mM sodium chloride, 2.4 mM potassium chloride, 0.04% polysorbate 20, 3 mM DTT, 10% glycerol, and variable imidazole

Purity: ≥80% estimated by SDS-PAGE

Formula Weight: 0

Shelf life (days): 180

Notes: JAK2 is a non-receptor tyrosine kinase that has roles in immune signaling.{19346,54923} It is composed of N-terminal FERM and SH2 domains, a regulatory JH2 pseudokinase domain, and a C-terminal JH1 kinase domain. It is widely expressed and associates with class I and class II cytokine receptors at the plasma membrane.{54921,54922} Activation of these cytokine receptors activates JAK2 and induces its dimerization and kinase activity, leading to JAK2 phosphorylation of STAT transcription factors and transcription of immune-related target genes. JAK2 signaling is inhibited by the suppressor of cytokine signaling (SOCS) proteins SOCS1 and SOCS3.{54924,54925} Gain-of-function mutations in JAK2 are associated with various blood disorders, including leukemias and myeloproliferative neoplasms.{54922} Cayman’s JAK2 JH1 and JH2 Domains (human, recombinant; aa 532-1,132) protein can be used for enzyme activity assays and has a calculated molecular weight of 98 kDa.

• Artikelnummer: CAY42272-20
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 42272-20
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Klonalität: Recombinant