JMJD2A (human, recombinant; Strep-tagged)

Formulation: 20 mM HEPES, pH 7.4, and 150 mM NaCl

Purity: ≥95% estimated by SDS-PAGE

Shelf life (days): 180

Notes: Methylation of lysine residues of core histones plays a critical role in the regulation of gene expression.{18003} Jumonji domain containing 2A (JMJD2A) is the first reported trimethyllysine-specific histone demethylase.{17969} It catalyzes the demethylation of trimethylated forms of histone at lysine residues 9 and 36. Like other JmjC protein hydroxylase family members, JMJD2A is an α-ketoglutarate-dependent Fe (II) oxygenase.{17970} Purification of Fe-dependent JmjC family members by IMAC can result in displacement of the catalytic iron and decreased activity, therefore this Strep-tagged protein is purified by affinity chromatography using Strep-Tactin coated resin.{20824} JMJD2A’s transcriptional function appears to depend on protein associations, as it is implicated in both transcriptional silencing and upregulation of the androgen receptor-dependent genes.{17969} Because of their implication in cancer cell growth, jumonji C domain-containing histone demethylases may be drug discovery targets for therapeutic intervention.{18003}

• Artikelnummer: CAY11299
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 11299-100
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Methode: Western Blotting