PAD1 (human, recombinant)

Formulation: 50 mM HEPES, pH 8.0, with 150 mM sodium chloride, 1 mM DTT, and 20% glycerol

Purity: ≥95% estimated by SDS-PAGE

Shelf life (days): 730

Notes: Peptidyl arginine deiminase 1 (PAD1) is a calcium-dependent enzyme that catalyzes the conversion of arginine residues to citrulline within its cellular protein substrates, resulting in the loss of a positive charge, which can alter protein structure and/or function.{45610,64948} It exists as a monomer and is composed of a C-terminal catalytic domain and two N-terminal immunoglobulin-like (Ig-like) domains.{64948} PAD1 is primarily expressed in uterine and epidermal cells and generally localized in the cytosol.{19490} PAD1 citrullinates non-histone proteins, such as keratin, filaggrin, and MEK1, as well as histone H3 at arginine 2 (H3R2), H3R8, H3R17, and H4R3, to regulate epidermal cell progression and embryonic development.{64949,64950,59195} The catalytic activity of PAD1 is lost by deleting the first six N-terminal amino acids.{64948} Decreased Padi1 expression or PAD1 inhibition reduces citrullination of H3R2, H3R8, H3R17, and H4R3 and inhibits primary mouse embryo preimplantation development.{59145} PADI1 mRNA is overexpressed in tumor tissue from patients with triple-negative breast cancer (TNBC), and PAD1 promotes proliferation and epithelial-to-mesenchymal transition (EMT) in a variety of TNBC cell lines in vitro and in mouse xenograft models.{64951} Cayman's PAD1 (human, recombinant) protein can be used for enzyme activity assay and Western blot (WB) applications.

• Artikelnummer: CAY10784
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 10784-100
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Methode: Western Blotting