PARP2 (human, recombinant)

Formulation: 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 16 mM glutathione, 20% glycerol, and 3 mM DTT

Purity: ≥50% estimated by SDS-PAGE

Shelf life (days): 180

Notes: Poly(ADP-Ribose) polymerase 2 (PARP2) is an ADP-ribosylating enzyme with a role in the DNA damage response (DRR).{61141,61142} It is composed of a natively disordered N-terminal region containing a nuclear localization sequence, a central Trp-Gly-Arg (WGR) domain that is essential to DNA damage-dependent catalytic domain activity, and a C-terminal catalytic domain (CAT) that contains an ADP-ribosyltransferase fold and a regulatory helical subdomain.{61142} PARP2 is ubiquitously expressed and localized to the nucleus where it is preferentially activated by the presence of PARP1-induced poly(ADP)-ribosylation (PARylation) induced by PARP1 (Item No. 32561) at sites of single-stranded DNA breaks. The WGR domain binds directly to DNA and activates PAR catalytic activity and branched PAR chain synthesis through cross-talk with the helical subdomain.{61141,61142} PARP2 PARylates itself and other proteins, including transcription factors, influencing multiple biological processes, such as transcription, genome maintenance, and cell signaling.{61143} PARP2 expression is negatively correlated with the amount of tumor immune infiltrate in tumor samples isolated from patients with osteosarcoma.{61144} Cayman’s PARP2 (human, recombinant) protein can be used for enzyme assay applications. This protein consists of 582 amino acids and has a calculated molecular weight of 95.5 kDa.

• Artikelnummer: CAY32562
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 32562-10
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Cell Line