PKM2 (human, recombinant)

Formulation: 40 mM Tris-HCl, pH 8.0, with 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol, and 3 mM DTT

Purity: ≥90% estimated by SDS-PAGE

Shelf life (days): 180

Notes: Pyruvate kinase (PK) is an enzyme that catalyzes the transfer of a phosphate group from phosphoenolpyruvate to ADP during aerobic glycolysis.{57358} PKM2 is an isoform of PK encoded by PKM in humans that is primarily expressed during embryonic development and localized to the cytosol. PKM2 expression is attenuated in adult tissues, however, it is reactivated in cancer cells during tumor development.{57358,57359,57361} It functions as a tetramer in the cytosol that is formed or dissociated in response to post-translational modifications and various allosteric regulators, such as fructose-1,6-bisphosphate, as well as the oxidation of its cysteine residues.{57360} It interacts with various tyrosine kinases, such as Bcr-Abl, A-RAF, and FGFR1, in a reciprocal interaction that regulates PKM2 enzyme kinetics and kinase signaling.{57359} PKM2 also functions as a dimer that can translocate to the nucleus and activate various transcription factors, including STAT3, NF-κB, and HIF-1α, to induce gene transcription.{57361,57359} Levels of PKM2 are increased in mesothelioma, osteosarcoma, and hepatomas, as well as ovarian, breast, gallbladder, and gastric cancers, and are associated with poor prognosis. Cayman’s PKM2 (human, recombinant) protein can be used for enzyme assay applications.

• Artikelnummer: CAY32564
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 32564-20
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK