SARS-CoV-2 nsp16 Methyltransferase

Formulation: Lyophilized from sterile 20mM Tris 150mM sodium chloride, pH 8.3, with 5% trehalose, 5% mannitol, and 0.01% tween-80

Purity: ≥85% as determined by SDS-PAGE

Shelf life (days): 365

Notes: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53462,53459,53461,55257,55258} The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors.{57176} SARS-CoV-2 nsp16 methyltransferase is a 2'-O-methyltransferase (2'-O-MTase) and has a role in 5'-end capping of viral mRNAs.{52793,52794} In coronaviruses, nsp16 forms a complex with nsp10 to methylate nascent mRNAs at the ribose 2'-O position, creating a Cap-1 structure that facilitates increased translation of viral mRNAs and reduced innate immune recognition by the host cell.{52794,52795} Deficiency of nsp16 in the related virus SARS-CoV reduces viral RNA synthesis by approximately 10-fold in vitro, and mutations in the nsp16 KDKE catalytic tetrad in mouse-adapted SARS-CoV attenuate the virus in vivo.{52794,52796} An nsp10-derived peptide inhibitor of the nsp16/nsp10 complex increases survival in a mouse model of infection with the coronavirus mouse hepatitis virus (MHV).{52795} Cayman's SARS-CoV-2 nsp16 Methyltransferase protein consists of 299 amino acids and has a calculated molecular weight of 33.46 kDa.

• Artikelnummer: CAY31816
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 31816-100
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK