sPLA2 (human, recombinant Type V)

Formulation: 50 mM Tris-HCl, pH 8.0, containing 100 mM sodium chloride, 50 mM calcium chloride, and 20% glycerol

Purity: ≥95% estimated by SDS-PAGE

Shelf life (days): 365

Notes: Phospholipase A2 (PLA2) catalyzes the hydrolysis of fatty acids at the sn-2 position of glycerophospholipids. PLA2 (Type V) is a secretory PLA2 (sPLA2) of approximately 14 kDa and is one of the isoforms in the growing list of the PLA2 enzyme family.{11139} This enzyme, rather than the sPLA2 (Type II), is responsible for arachidonic acid mobilization leading to prostaglandin production in macrophages and mast cells.{3506,4455,4053} Consistent with this role, sPLA2 (Type V) is associated with the golgi apparatus, nuclear envelope, and plasma membrane in mouse bone marrow-derived mast cells.{7627} sPLA2 (Type V) has been cloned from a variety of species including human, mouse, and rat.{7748,5754,7749} The enzyme is expressed in heart, lung, placenta, and spleen, as well as P388D1 macrophages and mast cells.{3506,4455,7749} The specific activity of Cayman’s sPLA2 (human, recombinant Type V) was established using 1,2-bis(heptanoylthio) Glycerophosphocholine (Cayman Item No. 62235) as the substrate. The reaction was inhibited by thioetheramide-PC (Cayman Item No. 62750).

• Artikelnummer: CAY10009563
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 10009563-25
• Green Labware: Nein
• Verpackungseinheit: 25 µg
• Mengeneinheit: STK
• Methode: Western Blotting