Formulation: 50 mM HEPES, pH 8.0, 150 mM sodium chloride, 20% glycerol
Purity: ≥85% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Succinyl-CoA synthetase from E. coli is responsible for the coupled hydrolysis of succinyl-CoA to the production of ATP (or GTP) in the substrate-level phosphorylation step of the citric acid cycle (TCA).{40019} This enzyme complex is formed as a heterotetramer, α2β2, where the two α subunits each contain the coenzyme A and phosphate binding sites, and the two β subunits each contain the succinate binding sites and provide nucleotide binding specificity. This enzyme is Mg2+-dependent.