Thioredoxin 1 (C61S/C72S mutant; human, recombinant)

Formulation: Lyophilized in 50 mM Tris-HCl, pH 7.5, with 1 mM EDTA

Purity: ≥85% estimated by SDS-PAGE

Shelf life (days): 180

Notes: Thioredoxin 1 (Trx1) is a thiol-disulfide oxidoreductase and part of the antioxidant thioredoxin system that is involved in the maintenance of cellular thiol redox homeostasis.{57135,57136,57138} It is ubiquitously expressed, localizes primarily to the cytoplasm with some nuclear localization, and is upregulated in and released from cells under conditions of oxidative stress.{57135,57137,57136} Trx1 contains two active site cysteine residues at positions 32 and 35 with additional cysteines at positions 62, 69, and 73.{57136} During the catalytic cycle, the active site cysteines are oxidized to a disulfide upon reduction of oxidized protein disulfide substrates and are subsequently restored to their reduced state by thioredoxin reductase (TrxR) and NADPH.{57135,57136} In the human recombinant enzyme, the initiating methionine is removed, and thus the positions of the amino acids are counted without the N-terminal methionine.{61516} Cysteine-to-serine substitutions at Cys61 (C61S) and Cys72 (C72S) in Trx1 (Trx1C61S/C72S) prevent oxidation-induced inhibition of protein disulfide reductase activity in Trx1.{20447} Cayman's Thioredoxin 1 (C61S/C72S mutant; human, recombinant) protein can be used for enzyme activity assays.

• Artikelnummer: CAY11520
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 11520-500
• Green Labware: Nein
• Verpackungseinheit: 500 µg
• Mengeneinheit: STK