Thioredoxin 1 (human, recombinant)

Formulation: 50 mM Tris-HCl, pH 7.8, with 150 mM sodium chloride, 10% glycerol, 1 mM EDTA, and 0.1 mM DTT

Purity: ≥95% estimated by SDS-PAGE

Shelf life (days): 365

Notes: Thioredoxin 1 (Trx1) is a thiol-disulfide oxidoreductase and part of the antioxidant thioredoxin system that is involved in the maintenance of cellular thiol redox homeostasis.{57135,57136,57138} It is ubiquitously expressed, localizes primarily to the cytoplasm with some nuclear localization, and is upregulated in and released from cells under conditions of oxidative stress.{57135,57136,57137} Trx1 contains two active site cysteine residues at positions 32 and 35, with additional cysteines at positions 62, 69, and 73.{57136} During the catalytic cycle, the active site cysteines are oxidized to a disulfide upon reduction of oxidized protein disulfide substrates and are subsequently restored to their reduced state by thioredoxin reductase (TrxR) and NADPH.{57135,57136} It regulates redox-sensitive transcription factors including NF-κB, p53, and the glucocorticoid receptor, as well as inhibits apoptosis through redox-sensitive binding and regulation of apoptosis signal-regulating kinase 1 (ASK1).{57136,57137} Exogenous administration of extracellular recombinant human Trx1 has anti-inflammatory effects in a variety of animal models.{57136} Trx1 levels are increased in a variety of human primary tumors, and increased tumor levels of Trx1 are associated with decreased survival in patients with colorectal cancer.{57137} Cayman's Thioredoxin 1 (human, recombinant) protein can be used for enzyme activity assay and Western blot (WB) applications.

• Artikelnummer: CAY31038
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 31038-100
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Methode: Western Blotting
• Wirt: Escherichia Coli