Acridine Orange base, CAS 494-38-2

Product Description: Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays[1][2][3].

Formula: C17H19N3

Citations: Chemosphere. 2023 Apr;320:138071./Research Square Print. December 12th, 2022./Acta Pharm Sin B. 2021 Feb 11./Adv Funct Mater. 2023 Apr 14./Cell Death Dis. 2020 Jul 30;11(7):607./Cell Death Dis. 2021 Jan 13;12(1):80./Cell Prolif. 2021 Dec 24;e13172./Clin Transl Med. 2023 Mar;13(3):e1229./Environ Toxicol. 2023 May 20./Eur J Med Chem. 2024 May 17:273:116507/Free Radic Biol Med. 2023 Sep 1;208:402-417./Int J Mol Med. 2023 Apr 7./Int J Mol Sci. 2023 Sep 5, 24(18), 13812./Microbiol Spectr. 2023 May 1;e0313822./Nat Commun. 2023 Jun 30;14(1):3877./SSRN. 2023 Mar 9./SSRN. 2023 Nov 15.

References: [1]Darzynkiewicz Z, et al. Differential staining of DNA and RNA. Curr Protoc Cytom. 2004 Nov;Chapter 7:Unit 7.3./[2]Mirrett S. Acridine orange stain. Infect Control. 1982 May-Jun;3(3):250-2. /[3]Yektaeian N, et al. Lipophilic tracer Dil and fluorescence labeling of acridine orange used for Leishmania major tracing in the fibroblast cells. Heliyon. 2019 Dec 18;5(12):e03073.

CAS Number: 494-38-2

Molecular Weight: 265.35

Research Area: Others

Solubility: 10 mM in DMSO

Target: Parasite

• Artikelnummer: MEXHY-D0952-100
• Hersteller: MedChemExpress
• Hersteller Artikelnummer: HY-D0952-100
• Green Labware: Nein
• Verpackungseinheit: 100 mg
• Mengeneinheit: STK