ConA Lectin (FITC)

Species: Jack Bean (Canavalia ensiformis)

Product Description: Concanavalin A is a lectin protein (MW 104kDa), homotetramer 26 kDa; originally extracted from the jack-bean, Canavalia ensiformis. It binds specifically to certain structures found in various sugars α-mannosyl and α-glucosyl residues in glycoproteins. It was the first lectin to be available on a commercial basis and is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities. It is also used to purify macromolecules in lectin affinity chromatography. Concanavalin A interacts with diverse receptors containing mannose carbohydrates (serum and membrane glycoproteins).ConA agglutinate strongly erythrocytes without being blood group specific. Normal cell re agglutinated after trypsinisation. ConA is not also a lymphocyte mitogen and reacts with many bacteria, like E. coli Dictyostelium discoideum et B. substilis, but also widely believed to be involved in the interaction between alpha-mannosyl oligosaccharides on the surface of the HIV virus and the human T cell lymphocyte.

Specificity/Sensitivity: alpha-linked mannose and glucose

Form: Liquid

Buffer (with preservative): 10mM Bicarbonate, 150mM NaCl, 0.1mM Calcium Chloride, 0.01mM Manganese Chloride (pH8.0). (Con A has an Isoelectric point of about pH4.5-5.5, requires calcium or manganese ions at each of its four saccharide binding sites; These ions should be used in buffer. DO NOT USE Phosphate buffer for dilution of this lectin as it will decrease the activity of lectin), 0.05% Sodium azide.

Concentration: 5 mg/ml (Please refer to the vial label for the specific concentration.)

Background: Concanavalin A is a lectin protein (MW 104kDa), homotetramer 26 kDa; originally extracted from the jack-bean, Canavalia ensiformis. It binds specifically to certain structures found in various sugars α-mannosyl and α-glucosyl residues in glycoproteins. It was the first lectin to be available on a commercial basis and is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities. It is also used to purify macromolecules in lectin affinity chromatography. Concanavalin A interacts with diverse receptors containing mannose carbohydrates (serum and membrane glycoproteins).ConA agglutinate strongly erythrocytes without being blood group specific. Normal cell re agglutinated after trypsinisation. ConA is a also a lymphocyte mitogen.ConA reacts with many bacteria, like E. coli Dictyostelium discoideum et B. substilis It is also widely believed to be involved in the interaction between alpha-mannosyl oligosaccharides on the surface of the HIV virus and the human T cell lymphocyte. Inhibiting/Eluting sugars: 200 α-mM α-methyl mannoside / 200 mM α-methyl glucoside mixture.
Carbohydrate-Binding Specificity of Con A: (Manα1,2Manα1,2Man > Manα1,2Man > α-Man > α-Glc > αGlcNAc


Region Sequence: Native Protein

ExpressionSystem: Native Protein

Conjugation: FITC

Full Name: Concanavalin A, Canavalia ensiformis

• Artikelnummer: GTX01505-1
• Hersteller: GeneTex
• Hersteller Artikelnummer: GTX01505-1
• Green Labware: Nein
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK
• Methode: Immunofluorescence, Immunocytochemistry