Peanut Lectin (PE)

Species: Arachis hypogaea

Product Description: Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells.

Application Note: ICC/IF: 1:50-1:200. FACS: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Specificity/Sensitivity: Carbohydrate-Binding Specificity of Peanut Lectin : Galβ1, 3 GalNAc>>GalNAc>Gal

Form: Liquid

Buffer (with preservative): 10mM Bicarbonate (pH8.2), 150mM NaCl, 0.1mM Calcium Chloride, 0.05% Sodium azide.

Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)

Background: Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit.

Inhibiting/Eluting sugars: 200 mM Galactose
Carbohydrate-Binding Specificity of PNA: Galβ1, 3 GalNAc>>>>>GalNAc>Gal


Region Sequence: Native Protein

ExpressionSystem: Native Protein

Conjugation: PE

Full Name: Peanut agglutinin lectin, Arachis hypogaea

• Artikelnummer: GTX01509-500
• Hersteller: GeneTex
• Hersteller Artikelnummer: GTX01509-500
• Green Labware: Nein
• Verpackungseinheit: 500 μg
• Mengeneinheit: STK
• Reaktivität: Human, Monkey (Primate)
• Methode: Immunofluorescence, Flow Cytometry, Immunocytochemistry