Recombinant Cynomolgus Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-6His)

Active protein: YES.

Activity: mmobilized Cynomolgus Siglec-15-His(Cat#PKSQ050096) at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 125 ng/ml.

Protein Construction: Recombinant Cynomolgus Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a 6His tag at the C-terminus.

Sequence: Phe20-Thr263.

Fusion tag: C-6His.

Accession: A0A2K5UY47.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Mol Mass: 27.1 kDa.

AP Mol Mass: 30-40 kDa.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, 150mMNaCl, 0.3% Chaps, 5% Trehalose, pH 7.4.

Background: Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO−-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.

• Artikelnummer: ELSPKSQ050096-50
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-PKSQ050096-50
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK
• Reaktivität: Cynomolgus Monkey (Primate)
• Methode: ELISA
• Wirt: Human