Recombinant Human Langerin/CD207 Protein (His Tag)

Protein Construction: Recombinant Human Langerin is produced by our Mammalian expression system and the target gene encoding Tyr64-Pro328(Val278Ala) is expressed with a 6His tag at the N-terminus.

Sequence: Tyr64-Pro328(Val278Ala).

Fusion tag: N-His.

Accession: Q9UJ71.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg as determined by the LAL method.

Mol Mass: 31.5 kDa.

AP Mol Mass: 30-40 kDa.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Background: Langerin (CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family. Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis. Langerin is necessary and sufficient for Birbeck granule formation. Human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Human langerin shares 68%, 62%, 71% aa identity with mouse, rat and bovine langerin ECD, respectively. Trimerization greatly increases the lectin binding affinity. Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions. Langerin also mediates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae.

• Artikelnummer: ELSPKSH033707-10
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-PKSH033707-10
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Reaktivität: Human
• Wirt: Human