UEA I Lectin (Biotin)

Species: Ulex europaeus

Product Description: The anti-H (O) hemagglutinating activity of Ulex europaeus has been used widely to confirm blood group O activity. UEA I binds to many glycoproteins and glycolipids containing α –linked fucose residue. The purified lectin appears to be a dimer of two distinct polypeptide chains associated by noncovalent forces. This lectin does not react with Lea active blood group substance. The native protein has pI=6.0-6.1 and exhibits a Molecular weight 60,000-68,000 during gel filtration on Sephadex column.

Specificity/Sensitivity: Fucose blood group H

Form: Liquid

Buffer (with preservative): 10mM Phosphate (pH7.6), 150mM NaCl, 0.1mM Calcium Chloride, 0.05% Sodium azide.

Concentration: 2 mg/ml (Please refer to the vial label for the specific concentration.)

Background: The anti-H (O) hemagglutinating activity of Ulex europaeus has been used widely to confirm blood group O activity. UEA I binds to many glycoproteins and glycolipids containing α –linked fucose residue. The purified lectin appears to be a dimer of two distinct polypeptide chains associated by noncovalent forces. This lectin does not react with Lea active blood group substance. The native protein has pI=6.0-6.1 and exhibits a Molecular weight 60,000-68,000 during gel filtration on Sephadex column. This lectin will hemagglutinate fresh blood group O cells at 2-5μg/ml.Inhibiting/Eluting sugars: 50-100 mM L-fucoseCarbohydrate-Binding Specificity of UEA I: L-Fuα1,2Galβ1,4GlcNAcβ1,6R

Region Sequence: Native Protein

ExpressionSystem: Native Protein

Conjugation: Biotin

Full Name: Ulex europaeus Lectin

• Artikelnummer: GTX01511-1
• Hersteller: GeneTex
• Hersteller Artikelnummer: GTX01511-1
• Green Labware: Nein
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK
• Reaktivität: Human
• Methode: Western Blotting, ELISA, Immunohistochemistry