Nucleophosmin (human, recombinant)

Formulation: 50 mM HEPES, pH 7.8, with 150 mM sodium chloride and 10% glycerol

Purity: ≥60% estimated by SDS-PAGE

Shelf life (days): 180

Notes: Nucleophosmin is a nucleolar phosphoprotein that is involved in diverse biological processes, including ribosome maturation, DNA repair, and mitotic spindle assembly.{69419} It is highly post-translationally modified and composed of an oligomerization domain, which contains two nuclear export signals, three acidic domains, an intrinsically disordered region, which contains two nuclear localization signals, and a C-terminal RNA-binding domain.{69419,69420} Nucleophosmin is ubiquitously expressed and shuttles between the nucleolus and cytoplasm, however, alternative splicing of NPM1 produces a variant that lacks the RNA-binding domain and nucleolar localization signal and is instead localized throughout the nucleus.{69421,69419,69420} Nucleophosmin has histone- and protein chaperone activity and plays a role in ribosome assembly and export, centrosome duplication, cell cycle control, the stress response, and embryogenesis.{69419} Knockdown of NPM1 inhibits protein synthesis and the nuclear export of the 40S, 60S, and 80S ribosomal subunits in vitro and knockout of NPM1 is embryonic lethal in mice.{68459,68460} Nucleophosmin fusion proteins have been found in patients with acute promyelocytic leukemia or non-Hodgkin's lymphoma.{68461,60149}

• Artikelnummer: CAY37621-100
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 37621-100
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Wirt: Escherichia Coli