Cereblon/DDB1/Cul4A/Rbx1 Complex Recombinant

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Encompassing Amino Acids: Cereblon: 2-442(end); DDB1: 1-1140(end); Cul4a: 2-759(end); Rbx1: 2-108(end)

Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: PROTAC ternary complex formation with BRD3 and cereblon was assessed using AlphaScreen™ technology using FLAG-acceptor beads and GSH-donor beads. Various amounts of Cereblon were incubated in 10-µl reaction with 100 nM dBET1 and 15 nM GST-BRD3 BPS #31033 at room temperature for one hour. After this, FLAG-acceptor beads and GSHdonor beads were added followed by detection of A-counts.

Description: Cereblon/DDB1/Cul4A/Rbx1 complex of human Cereblon (GenBank Accession No. NM_016302) a.a. 2-442(end) with N-terminal FLAG-tag and MW= 51 kDa, human DDB1 (GenBank Accession No. NM_001923), a.a. 1-1140(end) with N-terminal FLAG-tag and MW= 128 kDa, human Cul4A (GenBank Accession No. NM_003589), a.a. 2-759(end) with N-terminal His-tag and MW= 87 kDa, human Rbx1 (GenBank Accession No. NM_014248), a.a. 2-108(end) with N-terminal His-tag and MW= 13 kDa, co-expressed in a HEK293 expression system.

Format: Aqueous buffer solution

Formulation: 40 mm Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20 and 20% Glycerol.

Genbank: Cereblon: NM_016302; DDB1: NM_001923; Cul4a: NM_003589; Rbx1: NM_014248

Purity: ≥90%

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: Cereblon: N-terminal FLAG-tag; DDB1: N-terminal FLAG-tag; Cul4a: N-terminal His-tag; Rbx1: N-terminal His-tag

Uniprot: Cereblon:Q96SW2; DDB1: Q16531; Cul4a: Q13619; Rbx1: P62877

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Gang, Lu., et al., Science. 2014; 343(6168): 305-309.2. Zhu, Y.X., et al., Blood. 2011; 118: 4771-4779.

• Artikelnummer: BPS100329-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100329-1
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK