Nitrotyrosine Affinity Sorbent

Formulation: 200 µg of anti-Nitrotyrosine Monoclonal Antibody (Item No. 189542) coupled to protein A agarose; supplied as a 50% slurry in PBS, pH 7.2, with 0.02% sodium azide

Shelf life (days): 365

Notes: Nitrotyrosine is a post-translational modification that is formed by the nitration of tyrosine.{60009} It exists in a free or protein-bound form and is commonly used as a marker of nitrosative or oxidative stress.{31710} Nitrotyrosine residues have been found in a variety of proteins, including LDL, surfactant protein A, angiotensin II, and human and bovine serum albumin.{60010} Nitrotyrosine levels are increased in the affected tissues of numerous pathological conditions, including atherosclerosis, cancer, ulcerative colitis, Alzheimer's disease, and Parkinson's disease.{60010} Autoantibodies that recognize nitrotyrosinated proteins are increased in the synovium of patients with rheumatoid arthritis and are positively correlated with joint and tendon inflammation.{60009} Cayman's Nitrotyrosine Affinity Sorbent is designed for immunoprecipitation (IP) of nitrated proteins from biological samples. The nitrotyrosine affinity sorbent consists of Cayman's Nitrotyrosine Monoclonal Antibody (Item No. 189542) coupled to protein A agarose. This is an effective way to concentrate nitrated proteins for subsequent detection by a different anti-nitrotyrosine antibody by Western blot (WB).

• Artikelnummer: CAY389549
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 389549-200
• Green Labware: Nein
• Verpackungseinheit: 200 µg
• Mengeneinheit: STK
• Methode: Immunoprecipitation, Western Blotting