sFRP1 (human, recombinant)

Formulation: Lyophilized from sterile PBS, pH 7.4

Purity: ≥97% as determined by SDS-PAGE

Shelf life (days): 365

Notes: Secreted frizzled-related protein 1 (sFRP1) is a Wnt inhibitor and a member of the sFRP family.{66323,66324} It is composed of a signal peptide, a cysteine-rich domain, and a netrin-like domain.{66323} sFRP1 is ubiquitously expressed, with the highest levels in the heart, ovary, testis, and small intestine.{66325} It inhibits Wnt signaling by sequestering Wnt directly via the cysteine-rich or netrin-like domains or competing with Wnt for binding to the cysteine-rich domain of Frizzled (Fz) receptors.{66323} sFRP1 is involved in apoptosis, adipocyte differentiation, angiogenesis, osteoblast proliferation and differentiation, development, and hematopoietic stem cell maintenance.{66323,66324} Myocardial overexpression of Sfrp1 protects against decreases in cardiac function and increases in myocardium apoptosis in a mouse model of heart failure induced by transverse aortic constriction.{66326} Tumor protein levels of sFRP1 are decreased in early-stage breast cancer, which is associated with poor prognosis.{66327} Cayman’s sFRP1 (human, recombinant) protein can be used for cell-based assays. This protein consists of 294 amino acids, has a calculated molecular weight of 34 kDa, and a predicted N-terminus of Ser32 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 38 kDa due to glycosylation.

• Artikelnummer: CAY38061
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 38061-50
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK
• Methode: Cell-based Assay