13C20,15N10-Cyclic di-GMP (sodium salt)

Formulation: A solid

Formal Name: 2,9-bis(2-(amino-15N)-6-oxo-1,6-dihydro-9H-purin-9-yl-13C5-15N4)-3,10-dihydroxyoctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9]tetraoxa[2,8]diphosphacyclododecine-5,12-bis(olate)-2R,3R,3aS,7,7aR,9R,10R,10aS,14,14aR-13C10 5,12-dioxide, disodium salt

Purity: ≥98%

Formula Markup: [13C]20H22[15N]10O14P2 • 2Na

Formula Weight: 764,2

Shelf life (days): 1460

Notes: 13C20,15N10-Cyclic di-GMP is intended for use as an internal standard for the quantification of cyclic di-GMP (Item No. 17144) by GC- or LC-MS. Cyclic di-GMP is a second messenger in bacteria involved in diverse prokaryotic processes, including biofilm formation, motility, virulence, and cell cycling.{28201,28203} In eukaryotic cells, cyclic di-GMP is detected by and binds to the transmembrane protein stimulator of interferon genes (STING; Kd = 1.21 µM), leading to activation of the innate immune system.{28202,29220} It has been used at a preset molar ratio with STING dimers in binding assays to determine the binding constants of particularly tight binding partners, such as 2’3’-cGAMP. Cyclic di-GMP induces IFN-β mRNA expression in vitro (EC50 = 537.8 nM) but less potently than 2’3’-cGAMP (Item No. 19887), 3’2’-cGAMP, 3’3’-cGAMP (Item No. 17966), and 2’2’-cGAMP (Item No. 22419).

• Artikelnummer: CAY36245
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 36245-36
• Green Labware: Nein
• Verpackungseinheit: 36 µg
• Mengeneinheit: STK
• Methode: GC-MS, LC-MS, Isotopenmarkierung