PLPro Substrate, CAS 167698-69-3

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Amino Acid Sequence: Z-RLRGG-AMC

Applications: Useful as a substrate in the Papain-like Protease (SARS-CoV-2) Assay Kit: Protease Activity (BPS Bioscience #79995), as well for screening services.

CAS Number: 167698-69-3

Concentration: 5 mM in DMSO

Description: The PLPro (Papain-Like Protease) substrate is a small 5-mer fluorogenic peptide (Z- RLRGG-AMC) used to measure the activity of SARS family PLPro proteases. Release of the C-terminal AMC dye (7-amido-4-methylcoumarin ) by proteolysis generates fluorescence, which can be monitored at an excitation wavelength of 345 nm and an emission wavelength of 445 nm.Conditions optimized for use with: Papain-like Protease (SARS-CoV-2) Assay Kit: Protease Activity (BPS Bioscience #79995). The peptide corresponds to a short sequence of ubiquitin and may also be used as substrate of USP5 (isopeptidase T) and ubiquitin C-terminal hydrolases (UCH family, for example UCHL1).

Formulation: DMSO

Solubility: DMSO

Storage Stability: -20°C or -80°C

Supplied As: 5 mM in DMSO

Warnings: Avoid freeze/thaw cycles. Protect from light.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS79997
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 79997
• Green Labware: Nein
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK
• Methode: PS