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Mouse anti CD68 (Macrosialin), conjugated to FITC
Mouse anti CD68 (Macrosialin), conjugated to FITC, IgG1, Clone: Ki-M7
Artikelnummer
NORGM-4152
Verpackungseinheit
2ml (100 Tests)
Hersteller
Nordic-MUbio
Verfügbarkeit:
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Clone: Ki-M7
Label: FITC
Background: CD68 (macrosialin) is a type-one membrane protein with significant sequence homology to a family of lysosomal-associated glycoproteins (lamp). CD68 molecules are predominantly located intracellularily in cytoplasmic granules but can also be detected in smaller amounts at the cell surface. Particular strong intracellular expression is observed for monocytes and macrophages. In addition, Langerhans cells as well as plasmacytoid dendritic cells express clear-cut levels of CD68. Low-level reactivity is also observed with a subset of B lymphocytes and activated T lymphocytes. Oxidized low-density lipoprotein is a ligand for CD68. The CD68 mAb permits the identification and enumeration of monocytes, Langerhans cells and plasmacytoid dendritic cells (in combination with CD4/CD56 staining) in normal and malignant human blood and bone marrow samples using flow cytometry. Results must be interpreted by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us.
Purification Method: Purified by Chromatography
Specificity: The CD68 mAb (clone Ki-M7) reacts with human macrosialin. The sensitivity of CD68 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect identical percentage of cells). In practice, 50µl of leukocytes containing 10^7 cells/ml are stained with 20µl mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.
Formulation: PBS pH 7.2, 1% BSA, 0.05% NaN3
References: 1. Davey, F. R., Cordell, J. L., Erber, W. N., Pulford, K. A., Gatter, K. C. & Mason, D. Y. (1988) J Clin Pathol 41, 753-8. 2. Feuillard, J., Jacob, M. C., Valensi, F., Maynadie, M., Gressin, R., Chaperot, L., Arnoulet, C., Brignole-Baudouin, F., Drenou, B., Duchayne, E., Falkenrodt, A., Garand, R., Homolle, E., Husson, B., Kuhlein, E., Le Calvez, G., Sainty, D., Sotto, M. F., Trimoreau, F. & Bene, M. C. (2002) Blood 99, 1556-63.3. Fukuda, M. (1991) J Biol Chem 266, 21327-30. 4. Holness, C. L. & Simmons, D. L. (1993) Blood 81, 1607-13. 5. Knapp, W., Strobl, H. & Majdic, O. (1994) Cytometry 18, 187-98. 6. Pulford, K. A., Rigney, E. M., Micklem, K. J., Jones, M., Stross, W. P., Gatter, K. C. & Mason, D. Y. (1989) J Clin Pathol 42, 414-21. 7. Rabinowitz, S. S. & Gordon, S. (1991) J Exp Med 174, 827-36. 8. Ramprasad, M. P., Fischer, W., Witztum, J. L., Sambrano, G. R., Quehenberger, O. & Steinberg, D. (1995) Proc Natl Acad Sci U S A 92, 9580-4. 9. Ramprasad, M. P., Terpstra, V., Kondratenko, N., Quehenberger, O. & Steinberg, D. (1996) Proc Natl Acad Sci U S A 93, 14833-8. 10. Sadovnikova, E., Parovichnikova, E. N., Savchenko, V. G., Zabotina, T. & Stauss, H. J. (2002) Leukemia 16, 2019-26. 11. Scheinecker, C., Strobl, H., Fritsch, G., Csmarits, B., Krieger, O., Majdic, O. & Knapp, W. (1995) Blood 86, 4115-23. 12. Strobl, H. & Knapp, W. (2004) J Biol Regul Homeost Agents 18, 335-9. 13. Strobl, H., Scheinecker, C., Csmarits, B., Majdic, O. & Knapp, W. (1995) Br J Haematol 90, 774-82. 14. Strobl, H., Scheinecker, C., Riedl, E., Csmarits, B., Bello-Fernandez, C., Pickl, W. F., Majdic, O. & Knapp, W. (1998) J Immunol 161, 740-8
UniProt: P34810
Caution: For professional users only. This reagent contains sodium azide. To avoid the development of hazardous conditions, reagents containing azide should be diluted in running water prior to be discarded. Similar to the work with other biological products, proper handling procedures are recommended.
Label: FITC
Background: CD68 (macrosialin) is a type-one membrane protein with significant sequence homology to a family of lysosomal-associated glycoproteins (lamp). CD68 molecules are predominantly located intracellularily in cytoplasmic granules but can also be detected in smaller amounts at the cell surface. Particular strong intracellular expression is observed for monocytes and macrophages. In addition, Langerhans cells as well as plasmacytoid dendritic cells express clear-cut levels of CD68. Low-level reactivity is also observed with a subset of B lymphocytes and activated T lymphocytes. Oxidized low-density lipoprotein is a ligand for CD68. The CD68 mAb permits the identification and enumeration of monocytes, Langerhans cells and plasmacytoid dendritic cells (in combination with CD4/CD56 staining) in normal and malignant human blood and bone marrow samples using flow cytometry. Results must be interpreted by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us.
Purification Method: Purified by Chromatography
Specificity: The CD68 mAb (clone Ki-M7) reacts with human macrosialin. The sensitivity of CD68 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect identical percentage of cells). In practice, 50µl of leukocytes containing 10^7 cells/ml are stained with 20µl mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.
Formulation: PBS pH 7.2, 1% BSA, 0.05% NaN3
References: 1. Davey, F. R., Cordell, J. L., Erber, W. N., Pulford, K. A., Gatter, K. C. & Mason, D. Y. (1988) J Clin Pathol 41, 753-8. 2. Feuillard, J., Jacob, M. C., Valensi, F., Maynadie, M., Gressin, R., Chaperot, L., Arnoulet, C., Brignole-Baudouin, F., Drenou, B., Duchayne, E., Falkenrodt, A., Garand, R., Homolle, E., Husson, B., Kuhlein, E., Le Calvez, G., Sainty, D., Sotto, M. F., Trimoreau, F. & Bene, M. C. (2002) Blood 99, 1556-63.3. Fukuda, M. (1991) J Biol Chem 266, 21327-30. 4. Holness, C. L. & Simmons, D. L. (1993) Blood 81, 1607-13. 5. Knapp, W., Strobl, H. & Majdic, O. (1994) Cytometry 18, 187-98. 6. Pulford, K. A., Rigney, E. M., Micklem, K. J., Jones, M., Stross, W. P., Gatter, K. C. & Mason, D. Y. (1989) J Clin Pathol 42, 414-21. 7. Rabinowitz, S. S. & Gordon, S. (1991) J Exp Med 174, 827-36. 8. Ramprasad, M. P., Fischer, W., Witztum, J. L., Sambrano, G. R., Quehenberger, O. & Steinberg, D. (1995) Proc Natl Acad Sci U S A 92, 9580-4. 9. Ramprasad, M. P., Terpstra, V., Kondratenko, N., Quehenberger, O. & Steinberg, D. (1996) Proc Natl Acad Sci U S A 93, 14833-8. 10. Sadovnikova, E., Parovichnikova, E. N., Savchenko, V. G., Zabotina, T. & Stauss, H. J. (2002) Leukemia 16, 2019-26. 11. Scheinecker, C., Strobl, H., Fritsch, G., Csmarits, B., Krieger, O., Majdic, O. & Knapp, W. (1995) Blood 86, 4115-23. 12. Strobl, H. & Knapp, W. (2004) J Biol Regul Homeost Agents 18, 335-9. 13. Strobl, H., Scheinecker, C., Csmarits, B., Majdic, O. & Knapp, W. (1995) Br J Haematol 90, 774-82. 14. Strobl, H., Scheinecker, C., Riedl, E., Csmarits, B., Bello-Fernandez, C., Pickl, W. F., Majdic, O. & Knapp, W. (1998) J Immunol 161, 740-8
UniProt: P34810
Caution: For professional users only. This reagent contains sodium azide. To avoid the development of hazardous conditions, reagents containing azide should be diluted in running water prior to be discarded. Similar to the work with other biological products, proper handling procedures are recommended.
| Artikelnummer | NORGM-4152 |
|---|---|
| Hersteller | Nordic-MUbio |
| Hersteller Artikelnummer | GM-4152 |
| Verpackungseinheit | 2ml (100 Tests) |
| Mengeneinheit | PAK |
| Reaktivität | Human |
| Klonalität | Monoclonal |
| Methode | Flow Cytometry |
| Isotyp | IgG1 |
| Wirt | Mouse |
| Konjugat | Conjugated, FITC |
| Produktinformation (PDF) | Download |
| MSDS (PDF) | Download |
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