English
Mouse anti EpCAM/CD326/EGP-2/SCLC-CD2 epithelial antigen
Mouse anti EpCAM/CD326/EGP-2/SCLC-CD2 epithelial antigen, IgG2a, Clone: MOC-181
Artikelnummer
NORMUB0512P
Verpackungseinheit
0,1mg
Hersteller
Nordic-MUbio
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Clone: MOC-181
Background: The epithelial cell adhesion molecule (EpCAM), also known as CD326, is a 40 kD glycoprotein that is expressed at the basolateral cell membrane of simple, pseudo-stratified and transitional epithelia of normal adult tissues. EpCAM is reported to function as a homotypic calcium-independent cell adhesion molecule but studies suggest that EpCAM is also involved in cellular processes such as cell signalling, migration, proliferation and differentiation. EpCAM is overexpressed in a wide range of human epithelial carcinomas including lung, colorectal, breast, prostate, head and neck, and hepatic carcinomas. The EpCAM molecule has, therefore, been widely used as a diagnostic marker for human carcinomas and has been considered as a potential target for immunotherapy. Since EpCAM is also present on exosomes, microvesicles abundantly secreted by tumor cells, antibodies to this cell surface antigen are widely used for the isolation and identification of these cancer derived exosomes. Apart from EpCAM ́s role in cancer syndromes, homozygous mutations in the EpCAM gene have been identified in patients suffering from congenital tufting enteropathy, a rare autosomal recessive form of intractable diarrhea of infancy.Finally, EpCAM expression may be causally related to maintaining stem cells, and therefore will be a valuable target for future avenues to generate human induced pluripotent stem cells.
Source: MOC-181 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with an extract from a small cell lung carcinoma cell line.
Specificity: The antibody MOC-181 is directed against human EpCAM. The antibody has been reported to react with an epitope within the first EGF-like repeat in the extracellular region of EpCAM.
Formulation: Each vial contains 100µg 1mg/ml purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09% sodium azide.
References: 1. De Leij L, Helrich W, Stein R, Mattes MJ. 1994. SCLC-cluster-2 antibodies detect the pancarcinoma/epithelial glycoprotein EGP-2. Int J Cancer Suppl.8:60-3.2. 2. Kroesen BJ, McLaughlin PM, Schuilenga-Hut PH, Jacobs SC, Molema G, Helfrich W, De Leij LF. 2002. Tumor-targeted immune complex formation: effects on myeloid cell activation and tumor-directed immune cell migration.Int J Cancer.98:857-63.3. 3. Schnell U, Cirulli V, Giepmans BN.2013. EpCAM: structure and function in health and disease. Biochim Biophys Acta.1828:1989-2001
UniProt: UniProt: P16422 (Human)
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Background: The epithelial cell adhesion molecule (EpCAM), also known as CD326, is a 40 kD glycoprotein that is expressed at the basolateral cell membrane of simple, pseudo-stratified and transitional epithelia of normal adult tissues. EpCAM is reported to function as a homotypic calcium-independent cell adhesion molecule but studies suggest that EpCAM is also involved in cellular processes such as cell signalling, migration, proliferation and differentiation. EpCAM is overexpressed in a wide range of human epithelial carcinomas including lung, colorectal, breast, prostate, head and neck, and hepatic carcinomas. The EpCAM molecule has, therefore, been widely used as a diagnostic marker for human carcinomas and has been considered as a potential target for immunotherapy. Since EpCAM is also present on exosomes, microvesicles abundantly secreted by tumor cells, antibodies to this cell surface antigen are widely used for the isolation and identification of these cancer derived exosomes. Apart from EpCAM ́s role in cancer syndromes, homozygous mutations in the EpCAM gene have been identified in patients suffering from congenital tufting enteropathy, a rare autosomal recessive form of intractable diarrhea of infancy.Finally, EpCAM expression may be causally related to maintaining stem cells, and therefore will be a valuable target for future avenues to generate human induced pluripotent stem cells.
Source: MOC-181 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with an extract from a small cell lung carcinoma cell line.
Specificity: The antibody MOC-181 is directed against human EpCAM. The antibody has been reported to react with an epitope within the first EGF-like repeat in the extracellular region of EpCAM.
Formulation: Each vial contains 100µg 1mg/ml purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09% sodium azide.
References: 1. De Leij L, Helrich W, Stein R, Mattes MJ. 1994. SCLC-cluster-2 antibodies detect the pancarcinoma/epithelial glycoprotein EGP-2. Int J Cancer Suppl.8:60-3.2. 2. Kroesen BJ, McLaughlin PM, Schuilenga-Hut PH, Jacobs SC, Molema G, Helfrich W, De Leij LF. 2002. Tumor-targeted immune complex formation: effects on myeloid cell activation and tumor-directed immune cell migration.Int J Cancer.98:857-63.3. 3. Schnell U, Cirulli V, Giepmans BN.2013. EpCAM: structure and function in health and disease. Biochim Biophys Acta.1828:1989-2001
UniProt: UniProt: P16422 (Human)
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
| Artikelnummer | NORMUB0512P |
|---|---|
| Hersteller | Nordic-MUbio |
| Hersteller Artikelnummer | MUB0512P |
| Verpackungseinheit | 0,1mg |
| Mengeneinheit | STK |
| Reaktivität | Human |
| Klonalität | Monoclonal |
| Methode | Flow Cytometry |
| Isotyp | IgG2a |
| Wirt | Mouse |
| Konjugat | Unconjugated |
| Produktinformation (PDF) | Download |
| MSDS (PDF) | Download |
Verwandte Artikel
Markieren Sie die Artikel, um Sie dem Warenkorb hinzuzufügen oder
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
