Mouse anti Vimentin, conjugated with HRP

Mouse anti Vimentin, conjugated with HRP, Monoclonal, IgG1, Clone: RV202

Clone: RV202

Label: HRP

Background: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Source: RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Specificity: RV202-HRP reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchyme derived tumors e.g. lymphoma, sarcoma and melanoma. RV202-HRP is suitable for immunoblotting, immunocytochemistry on cell cultures and cytospins, as well as immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended dilution is 1:10.

References: 1. Ramaekers, F., Huysmans, A., Schaart, G., Moesker, O., and Vooijs, P. (1987). Tissue distribution of Keratin 7 as monitored by a monoclonal antibody, Exp Cell Res 170, 235-49. 2. Viebahn, C., Lane, E. B., and Ramaekers, F. C. (1988). Keratin and vimentin expression in early organogenesis of the rabbit embryo, Cell Tissue Res 253, 553-62. 3. Pieper, F. R., Schaart, G., Krimpenfort, P. J., Henderik, J. B., Moshage, H. J., van de Kemp, A., Ramaekers, F. C., Berns, A., and Bloemendal, H. (1989). Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells, J Cell Biol 108, 1009-24. 4. Raats, J. M., Pieper, F. R., Vree Egberts, W. T., Verrijp, K. N., Ramaekers, F. C., and Bloemendal, H. (1990). Assembly of amino-terminally deleted desmin in vimentin-free cells, J Cell Biol 111, 1971-85. 5. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55.

UniProt: P08670

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

• Artikelnummer: NORMUB1900L2
• Hersteller: Nordic-MUbio
• Hersteller Artikelnummer: MUB1900L2
• Green Labware: Nein
• Verpackungseinheit: 1 ml
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine), Rat (Rattus), Pig (Porcine), Monkey (Primate), Dog (Canine), Hamster, Zebrafish, Chicken, Goat (Caprine), Frog (Xenopus Laevis)
• Klonalität: Monoclonal
• Methode: Immunohistochemistry (frozen), Flow Cytometry, Immunocytochemistry
• Isotyp: IgG1
• Wirt: Mouse