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Avidin Peroxidase Conjugated
Avidin Peroxidase Conjugated
Artikelnummer
ROCA003-03
Verpackungseinheit
2 mg
Hersteller
Rockland
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Application Note: Avidin Peroxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Peroxidase Conjugated can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).
Concentration Value: 2.0 mg/mL
ELISA Dilution: 1:20,000 - 1:200,000
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Western Blot Dilution: 1:10,000 - 1:40,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Lyophilized
Purity and Specificity: Avidin Peroxidase Conjugated was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Avidin Peroxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin and anti-Peroxidase.
Background: Avidin is a biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the greatest known non-covalent interactions. Avidity for biotin is destroyed with heat. Horseradish Peroxidase (HRP) is an enzyme that utilizes organic peroxide compounds as electron donors. Horseradish Peroxidase naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Avidin Peroxidase Conjugated is ideal for investigators in Immunology, Cancer, Neuroscience, and Cell Biology.
Low Endotoxin: No
Other: User Optimized
Concentration Value: 2.0 mg/mL
ELISA Dilution: 1:20,000 - 1:200,000
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Western Blot Dilution: 1:10,000 - 1:40,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Lyophilized
Purity and Specificity: Avidin Peroxidase Conjugated was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Avidin Peroxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin and anti-Peroxidase.
Background: Avidin is a biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the greatest known non-covalent interactions. Avidity for biotin is destroyed with heat. Horseradish Peroxidase (HRP) is an enzyme that utilizes organic peroxide compounds as electron donors. Horseradish Peroxidase naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Avidin Peroxidase Conjugated is ideal for investigators in Immunology, Cancer, Neuroscience, and Cell Biology.
Low Endotoxin: No
Other: User Optimized
| Artikelnummer | ROCA003-03 |
|---|---|
| Hersteller | Rockland |
| Hersteller Artikelnummer | A003-03 |
| Verpackungseinheit | 2 mg |
| Mengeneinheit | STK |
| Konjugat | Conjugated, HRP |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
