Streptavidin (AP)

Product Description: Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Protein content: > 95%; Alkaline Phosphatase (ALP) used in this conjugation is 140 KDa enzyme purified from calf intestine.

Form: Liquid

Buffer (with preservative): 10mM Tris-HCl (pH8.0), 200mM NaCl, 50% Glycerol, 10 mg BSA, 0.05% Sodium azide.

Concentration:

Background: Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band;Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Alkaline Phosphatase (ALP) used in this conjugation is 140 KDa enzyme purified from calf intestine.

Conjugation: AP

• Artikelnummer: GTX85909-500
• Hersteller: GeneTex
• Hersteller Artikelnummer: GTX85909-500
• Verpackungseinheit: 500 μl
• Mengeneinheit: STK
• Methode: Western Blotting, ELISA, Immunohistochemistry