Application Note: UniGlow™ is a highly sensitive, nonradioactive, enhanced luminol-based, chemiluminescent, ready-to-use peroxidase (HRP) substrate and no mixing is required. UniGlow™- One Component Chemiluminescent Substrate is for use in microwell or membrane applications. Protect from light - UniGlow™ Substrate is a highly sensitive detection reagent allowing for the detection of picograms (6-12 pg) amounts of antigen. Equilibrate to room temperature before use and aliquot into a clean container. Use ~100µL/cm2 of membrane. Optimal detection visualized after contact of substrate with HRP enzyme on membrane for ~1-10min. Blot off excess substrate before imaging. Always carefully optimize all components of individual assays (antigens, antibodies, conjugates…) to minimize background reactivity associated with non-specific binding.
Concentration Value: 1X
ELISA Dilution: 1X
Western Blot Dilution: 1X
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Liquid - light red colored
Background: Enhanced Chemiluminescence (ECL) involves the enzymatic oxidation of luminol, a process that is catalyzed by peroxidase (HRP) enzyme. In the presence of HRP and a peroxide, luminol is oxidized, and emits light. The intensity of the emitted light is proportional to the amount of HRP-bound target, allowing for the quantitative detection of proteins.
Low Endotoxin: No
Other: User Optimized
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