Application Note: ABTS ELISA Peroxidase Substrate is a ready to use substrate - No dilution required. Read absorbance of blue green color at 405 nm. The color formation as a function of time can be recorded at intervals or the reaction may be stopped by addition of acid after 30 mins. A 0.625 Mol L-1 Oxalic Acid stop solution is recommended, color is stable for 1hr.
Concentration Value: 1X
ELISA Dilution: 1X
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Liquid - clear to very pale green
Purity and Specificity: pH: 4.0 +/- 0.05Stability @ 18° to 26°C: PASSStability @ 4°C: PASSQC Raw Material: PASSAbsorbance check of final product: PASSPerformance Data per ELISA: PASS
Background: ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) is a colorimetric substrate suitable for peroxidase (HRP) enzyme labeling, producing a green color upon oxidation that is measurable at 405-410 nm. ABTS is preferred for applications requiring a soluble end product and is compatible with both plate readers and spectrophotometers.
Low Endotoxin: No
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