The Trichrome Stain Kit (with Iodine) is intended for use in the histological visualization and differentiation of collagen in tissue sections. Gram's Iodine is offered as an alternative to Bouin's Fluid post fixative, and is significantly less hazardous. Iodine post-fixation improves the color and staining of collagen and is comparable to trichrome kits that utilize Bouin's. No nuclear counterstain is included with this kit. This kit is intended for use on formalin-fixed sections.